Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pan Chu
Date created: 2016-08-04 11:00:00
Date modified: 2016-10-09 01:16:18
c-di-GMP tandem riboswitche bc3-5
| Types | DnaRegion |
| Roles | Inverter engineered_region |
| Sequences | BBa_K2012000_sequence (Version 1) |
Description
Three complete c-di-GMP riboswitches (Bc3, Bc4 and Bc5 RNA) with similar structures, which are arranged in tandem to constitute a triple-tandem (Bc3-5 RNA) riboswitch in the 5′-UTR of the cspABCDE mRNA in Bacillus thuringiensis subsp. chinensis CT-43.Notes
We designed the sequence of bc3-5 by add perfix and suffix which are belong to BioBrick RFC 10.Source
From Bacillus thuringiensis subsp. chinensis CT-43In the CT-43 genome19,20 (GenBank accession: NC_017208.1), five type I c-di-GMP riboswitches named Bc1, Bc2, Bc3, Bc4 and Bc5 RNA (Supplementary Tables S1???S5) were annotated in the Rfam database (http://rfam.sanger.ac.uk/search).
Zhou, H., et al. (2016). "Characterization of a natural triple-tandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter." Sci Rep 6: 20871.