Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 10:22:03
AraC-pBAD->mRFP_W
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2014008_sequence (Version 1) |
Description
We were using standard AraC-pBAD promoter to compare the influence of different ORFs structures coding for the same protein mRFP.Notes
We were looking for the best method to optimize gene expression in E.coli.Source
The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the mRFP permutation, with optimized codon sequence.| Sequence Annotation | Location | Component / Role(s) |
| O2 region O1 region i1-i2 region -10 signal 5'UTR AraC CDS ht-mRFP-W CAP binding site -35 signal | 909,924 1066,1087 1113,1155 1177,1181 1191,1217 1,879 1218,1928 1111,1121 1153,1157 | operator feature/operator operator feature/operator non_covalent_binding_site feature/binding sequence_feature feature/misc sequence_feature feature/misc CDS feature/cds feature/protein CDS feature/binding non_covalent_binding_site feature/misc sequence_feature |