Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicola Freyer, Lea Steinbeck, Svenja Meyer, Alexander Deitert
Date created: 2016-10-03 11:00:00
Date modified: 2016-10-04 07:47:20
subtilisin E gene with leader sequence pelB
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2020001_sequence (Version 1) |
Description
This composite part consists of the leader sequence pelB (BBa_J32015) and the subtilisin E gene (BBa_K2020000). It can be used to express subtilisin E in Escherichia coli and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.Notes
The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT. Moreover, the leader sequence pelB begins with a start odon and was placed in front of the subtilisin E gene to generate a functional coding sequence that can be expressed in E. coli.Source
The sequence of the signal peptide pelB was obtained through the Registry (BBa_J32015: http://parts.igem.org/Part:BBa_J32015). Together with the subtilisin E gene (BBa_K2020000: http://parts.igem.org/Part:BBa_K2020000) it was ordered from IDT.| Access | Instance | Definition |
| public public | BBa_J32015 BBa_K2020000 | PelB subE |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J32015 BBa_K2020000 | 1,66 67,1125 | PelB subE |