BBa_K2020051

BBa_K2020051 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2020051
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher
Date created: 2016-10-14 11:00:00
Date modified: 2016-12-02 08:24:21

wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K2020051_sequence (Version 1)

    Description

    This is a DMNBS-synthetase to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate DMNBS in response to an amber stop codon.

    Notes

    The synthetase was created via mutation library following computational design from Methanococcus janaschii wild type tyrosyl synthetase.

    Source

    Basis for mutation was Methanococcus janaschii wild type tyrosyl synthetase.

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2020051/1
     

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