Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gavin Sutton
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-08 02:47:48
TolR, Periplasmic Domain
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2022002_sequence (Version 1) |
Description
Residues 44-117 of the tolR protein from E. coli, with added start and double stops. Overexpression of this part is known to stimulate formation of outer-membrane vesicles (OMVs) (Henry et al., 2004).Notes
As the sequence came from E. coli's genome, evolution has likely already made it codon optimised. However, this is only a single domain of the protein, hence start and stop codons had to be artificially added to ensure it is a proper coding sequence. A previous team attempted to submit this part to the registry (BBa_K257005, by Paris 2009), but forgot to add the start codon; we thus submit this part to amend that error.Another improvement we made was to add a second stop codon at the c-terminus, to more stringently terminate translation.