Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gordon Sun
Date created: 2016-10-23 11:00:00
Date modified: 2016-10-24 02:34:04
E. coli DXS Synthase Enzyme cds (codon optimized)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2027056_sequence (Version 1) |
Description
This sequence codes for DXS synthase, an enzyme involved in the conversion of G3P and Pyruvate to DXP in the MEP/DOXP pathway. Because DXS synthase activity is the rate limiting step in the MEP/DOXP pathway, overexpression of this gene will override the bottleneck in the G3P and Pyr conversion process.This sequence is endogenous to E. coli and is referenced from EcoGene:EG13612.
Notes
This sequence has been codon optimized for expression in E. coli.No further modifications were made.
Source
Sequence is sourced from Zhao Y, Yang J, Qin B, et al. Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway. Appl Microbiol Biotechnol. 2011;90(6):1915-22. (http://www.ecogene.org/gene/EG13612/dnasequence?q=gene/EG13612/dnasequence&sites[0]=BamHI&sites[1]=EcoRI&sites[2]=HindIII&left_end=433315&right_end=443315&type=fasta)EcoGene: EG13612