BBa_K203099

BBa_K203099 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K203099

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lars Velten, Simon Haas, Anne Rademacher, Hannah Meyer and Michael Bartoschek

Date created: 2009-10-14 11:00:00

Date modified: 2015-05-08 01:11:22

pSMB_REFERENCE

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid
• Sequences: BBa_K203099_sequence (Version 1)

Description

Can be used for normalizations to transfection efficiency during promoter measurements in mammalian cells, if pSMB_MEASURE is used for measurements.

Notes

mCherry was PCR amplified and replaced GFP in pSMB_MEASURE by cutting with BclI and PstI

Source

Modified from Part:BBa_K203100 to contain mcherry (cloned from a plasmid containing mcherry-N1) instead of GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mcherry	373,1083	feature/protein, CDS
SV40 terminator	2815,2934	feature/polya, polyA_site
Hygromycine Resistance	1672,2679	feature/protein, CDS
FRT site	1605,1652	sequence_feature, feature/misc
JeT	188,366	promoter, feature/promoter
Amp resistance	4143,5003	feature/protein, CDS
AmpR promoter	5045,5073	feature/promoter, promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K203099/1

Member of these Collections

• iGEM Parts Registry