DnaK

BBa_K205002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K205002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael James George Milevskiy

Date created: 2009-09-27 11:00:00

Date modified: 2015-05-08 01:11:23

DnaK is a chaperone protein that assists with polypeptide folding

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K205002_sequence (Version 1)

Description

In conjuction with DnaJ and GrpE, DnaK assists in correct polypeptide folding and is the Prokaryotic homologue of the Eukaryotic chaperone system 'Hsp70'. This gene, along with DnaJ, GrpE, GroEL and GroES forms the heat shock system in bacteria.

Notes

In order to comply with Standard 23, two restriction sites were removed within the gene. These sites were EcoRI and BglII, which were removed through mutagenesis.

Source

Christos Kyratsous Columbia Univ, Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA

Ref: Kyratsous, CA, Silverstein, SJ, DeLong, CR, Panagiotidis, CA. (2009) "Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli" GENE VOL: 440 Issue: 1-2 Pages: 9-15 Date: Jul 1 2009

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start of DnaK	1,3	feature/start, start_codon
End of DnaK	1897,1899	stop_codon, feature/stop
Mutation from t to g to remove the EcoRI restriction site	730,730	point_mutation, feature/s_mutation
Mutation from t to c to remove the BglII restriction site	933,933	point_mutation, feature/s_mutation

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K205002/1

Member of these Collections

• iGEM Parts Registry