BBa_K2060000

BBa_K2060000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2060000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cardiff_Wales Team, Geraint Parry

Date created: 2016-09-22 11:00:00

Date modified: 2016-09-30 06:06:30

CRISPR-Cas9 guide RNA targeting to 16S RNA

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K2060000_sequence (Version 1)

Description

This part includes a guide RNA that will target Cas9 to the E.Coli 16S ribosomal RNA locus. The guide RNA is expressed with the Cas9-recognised RNA scaffold. Expression of the guideRNA is driven by the T7 promotor with Lac0 binding site and followed by a T7 terminator sequence.

Notes

Used standard design considerations for guide RNAs

Source

E.coli 16S ribosomal RNA

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7	1,17	feature/promoter, promoter
LacO	20,44	feature/misc, sequence_feature
KpnI	45,50	feature/scar
guideRNA	51,152	feature/dna, sequence_feature
T7term	153,188	sequence_feature, feature/misc
guideRNA	51,53	start_codon, feature/start

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2060000/1

Member of these Collections

• iGEM Parts Registry