BBa_K2066502

BBa_K2066502 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2066502
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph L Maniaci
Date created: 2016-08-24 11:00:00
Date modified: 2016-08-25 07:55:30

1xTetO Binding Site



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K2066502_sequence (Version 1)

Description

This is an intermediate part used in the construction of WM16 TetO ICA parts (WM16_K2066002-WM16_K2066010). This part contains the TetO repeat sequence used by WM for ICA creation of TetO binding arrays. Tet Operator Sequence based off of Addgene Plasmid #17655. ICA design method based off of Briggs, A. W., Rios, X., Chari, R., Yang, L., Zhang, F., Mali, P., & Church, G. M. (2012). Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers. Nucleic acids research, gks624.

Notes

n/a

Source

This part was synthesized as part of a gBlock / using IDT DNA synthesis. Tet Operator Sequence based off of Addgene Plasmid #17655.

igem#status
Planning
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K2066502/1