BBa_K2080001

BBa_K2080001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2080001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haojian Li
Date created: 2016-09-21 11:00:00
Date modified: 2016-09-22 07:37:24

bax-sgRNA1



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K2080001_sequence (Version 1)

Description

This guide RNA is a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.

Notes

We design the sequence of the gRNA that is complementary to the bax promoter region, and try to get closer to the transcription start site to obtain higher activation of bax trascription.

Source

Form Bba_K2080001

Sequence Annotation Location Component / Role(s)
U6 promoter
gRNA for Bax promoter and Bax-1 promoter
gRNA scaffold
1,264
265,284
285,367
feature/promoter promoter
sequence_feature feature/misc
feature/misc sequence_feature
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2080001/1