BBa_K2100009

BBa_K2100009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2100009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathleen Brandes
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 09:57:26

pENTR pPRE4



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K2100009_sequence (Version 1)

Description

description here.

Notes

This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

This is a synthetic promoter from IDT.

Sequence Annotation Location Component / Role(s)
minCMV
PRE site
PRE site
PRE site
PRE site
184,241
50,65
121,136
86,101
157,172
promoter feature/promoter
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2100009/1