Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wangui Mbuguiro
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-19 04:46:33
Sensor 20A
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K2100051_sequence (Version 1) |
Description
This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.Notes
Synthesized by Jeremy Gam of MIT's Weiss Lab.Source
human genome| Sequence Annotation | Location | Component / Role(s) |
| miRNA-20A target site x4 mKate hEF1A hEF1A EBFP | 5,92 10268,10972 9030,10203 1461,2634 2690,3409 | sequence_feature feature/misc feature/protein CDS promoter feature/promoter promoter feature/promoter feature/protein CDS |