BBa_K2100056

BBa_K2100056 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2100056
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Goupil
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-26 04:39:34

Sensor 181A



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K2100056_sequence (Version 1)

Description

This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.

Notes

Courtesy of Jeremy Gam of MIT's Weiss Lab

Source

Human genome

Sequence Annotation Location Component / Role(s)
miR-181A-target site
hEF1A
eBFP
hEF1A
mKate
1,88
1465,2638
2694,3413
9034,10207
10272,10976
sequence_feature feature/misc
feature/promoter promoter
CDS feature/protein
promoter feature/promoter
feature/protein CDS
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2100056/1