BBa_K2100062

BBa_K2100062 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K2100062

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Trinh Nguyen

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-20 12:10:03

pEXPR EGSH: TP901

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA

Description

This is a final expression vector that we used to transfect into mammalian cells for our experiments. TP901 is a serine recombinase, which we used as biological latches to control expression of a final out put gene for our genetic circuit. pEGSH is an inducible promoter similar to pTRE. This inducible promoter system require the presence of VgEcr and RXR protein and small molecule Ponasterone A (PonA). By putting TP901 recombinase under pEGSH, we can control the expression of TP901 with small molecule PonA.

Notes

We used LR reaction to put to clone the promoter and gene to a backbone vector

Source

[[Part:BBa_K2100021|pENTR pEGSH]]
[[Part:BBa_K2100019|pENTR TP901]]

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2100062/1

Member of these Collections

• iGEM Parts Registry