BBa_K210013

BBa_K210013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K210013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Makoto Kashima
Date created: 2009-10-14 11:00:00
Date modified: 2015-05-08 01:11:29

LacI binding sites' tandem sequence #700



Types
DnaRegion

Roles
DNA

sequence_feature

Description

This is a tandem sequence of lacI binding sites(GAAATGTGAGCGCTCACAACT). In the project "Time bomb" of iGEM Kyoto 2009, this part was used in order to stbilize linear DNA.

It is very difficult to clone this parts because tandem sequence can induce mutation. So, if you want to use this parts, you should cloning this under 32C under which it is liower possible that mutation happens.

Notes

This parts was made by MPR.
Due to mutation, we could not suceed to sequence.
We confirmed this has only one set of EcoR1, Xba1, Spe1 and Pst1 recognition sites.

Source

primer

igem#sampleStatus
No part sequence
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K210013/1