BBa_K2114001

BBa_K2114001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2114001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wladislaw Stroukov

Date created: 2016-10-05 11:00:00

Date modified: 2016-10-19 02:50:21

aGFPnano_HA_aHelix_cotZ

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2114001_sequence (Version 1)

Description

N-terminal fusion of anti-GFP nanobody to spore crust gene cotZ.

Notes

Introduction of HA epitope tag and alpha helical linker by primer extensions.

Source

Amplification of cotZ from genomic DNA of Bacillus subtilis. The anti-GFP nanobody was provided by Dr. Maximilian Ulbrich (BIOSS)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
anti-GFP nanobody	1,354	feature/cds, CDS
HA	355,381	tag, feature/tag
alpha helical linker	382,414	feature/misc, sequence_feature
cotZ	415,861	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2114001/1

Member of these Collections

• iGEM Parts Registry