BBa_K2114002

BBa_K2114002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2114002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wladislaw Stroukov

Date created: 2016-10-05 11:00:00

Date modified: 2016-10-19 03:31:55

aGFPnano_HA_G4S_cotZ

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2114002_sequence (Version 1)

Description

N-terminal fusion of anti-GFP nanobody to spore crust gene cotZ by a flexible GGGGS linker.

Notes

Introduction of a HA epitope tag and a flexible GGGGS linker by primer extensions.

Source

The cotZ gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived camelid heavy-chain antibodies.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
anti-GFP nanobody	1,354	feature/cds, CDS
HA	355,381	tag, feature/tag
GGGGS linker	382,396	sequence_feature, feature/misc
cotZ	397,843	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2114002/1

Member of these Collections

• iGEM Parts Registry