BBa_K2114006

BBa_K2114006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2114006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wladislaw Stroukov

Date created: 2016-10-07 11:00:00

Date modified: 2016-10-10 08:36:01

CotZ_G4S_HA_GST

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2114006_sequence (Version 1)

Description

C-terminal fusion of glutathione S-transferase to spore crust gene cotZ by a flexible GGGGS linker.

Notes

Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

Source

The cotZ gene comes from the genome of B. subtilis. The glutathione S-transferase was amplified from the pGEX-6P-1 vector (GE healthcare).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cotZ	1,444	CDS, feature/cds
GGGGS linker	445,459	feature/misc, sequence_feature
HA	460,486	tag, feature/tag
Glutathione S-transferase	487,1188	CDS, feature/cds

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2114006/1

Member of these Collections

• iGEM Parts Registry