BBa_K2114011

BBa_K2114011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2114011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wladislaw Stroukov

Date created: 2016-10-07 11:00:00

Date modified: 2016-10-19 03:34:59

CotG_G4S_HA_GST

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2114011_sequence (Version 1)

Description

C-terminal fusion of glutathione S-transferase to spore crust gene cotG by a flexible GGGGS linker.

Notes

Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.

Source

The cotG gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cotG	1,585	feature/cds, CDS
GGGGS linker	586,600	feature/misc, sequence_feature
HA	601,627	feature/tag, tag
glutathione S-transferase	628,1329	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2114011/1

Member of these Collections

• iGEM Parts Registry