BBa_K2114019

BBa_K2114019 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114019
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-18 07:19:28

GST_HA_G4S_CgeA



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K2114019_sequence (Version 1)

    Description

    N-terminal fusion of glutathione S-transferase to spore crust gene cgeA by a flexible GGGGS linker.

    Notes

    Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

    Source

    The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).

    Sequence Annotation Location Component / Role(s)
    GST
    HA
    GGGGS linker
    cgeA
    		1,699
    700,726
    727,741
    742,1143
    		CDS feature/cds
    feature/tag tag
    feature/misc sequence_feature
    CDS feature/cds

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2114019/1
     

    Attachments

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