BBa_K2116027

BBa_K2116027 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2116027
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Asli Azizoglu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 07:07:08

Nitric oxide and Lactate responsive AND gate



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K2116027_sequence (Version 1)

Description

This part is a hybrid promoter, which consists of pNorV promoter between two LldO binding sites. pNorV is a nitric oxide responsive promoter, regulated by NorR. LldO binding sites enable binding of LldR repressor. The promoter exhibits an AND logic and is responsive to L-lactate and nitric oxide. It will be active when both two compounds are present.

Notes

...

Source

PnorV E.coli

Sequence Annotation Location Component / Role(s)
llDrO1
NorR binding 2 (low affinity)
NorR binding 1 (high affinity)
sigma 54 binding site
lldRO2
1,18
180,196
207,227
269,301
302,316
operator feature/operator
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
operator feature/operator
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2116027/1