Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elias Scheer
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:29
secretion signal peptide + GFPmut3b
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K212004_sequence (Version 1) |
Description
This is a composite signalling part. The secretion signal peptide for beta-lactamase in S. epidermidis is ligated N-terminally to registry part BBa_E0040 (GFPmut3b). The signal peptide motif is used by the bacteria to target the protein for secretion through the Sec pathway. Any part may be put in place of GFP, but in its current state, the amount of secretion of any protein may be studied using confocal microscopy.NOTE: This part only works for S. epidermidis ATCC 12228
Notes
Not Applicable.Source
This part was synthesized based on a known sequence, which may be found here: .| Sequence Annotation | Location | Component / Role(s) |
| secretion signal peptide GFPmut3b | 23,111 118,836 | non_covalent_binding_site feature/binding engineered_region feature/BioBrick |