BBa_K2130000

BBa_K2130000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2130000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ooi Kean Hean

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-22 08:40:43

∆
RuvCIII-2 ∆
HNH Sp-dCas9

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2130000_sequence (Version 1)

Description

dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner.
For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta.

http://2016.igem.org/wiki/images/0/0b/T--NTU-Singapore--ruvc3.jpg

http://2016.igem.org/wiki/images/a/a2/T--NTU-Singapore--ruvc3delresult.png

Notes

Created with Gibson

Source

Addgene

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GGGS linker	2327,2338	feature/misc, sequence_feature
(GGGS)2 linker	2618,2641	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2130000/1

Member of these Collections

• iGEM Parts Registry