BBa_K2132005

BBa_K2132005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2132005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: YIFAN CHEN

Date created: 2016-10-08 11:00:00

Date modified: 2016-10-09 05:45:15

HydA with SpyTag, Histag and TEV site

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2132005_sequence (Version 1)

Description

This is a modified protein, we added Histag and TEV site to the N-terminal site of hydA, and SpyTag to the C-terminal site of hydA.

Notes

We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

Source

The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SpyTag	1816,1857	feature/tag, tag
6x Histag	7,24	feature/tag, tag
TEV site	25,45	feature/misc, sequence_feature
HydA	46,1791	CDS, feature/cds

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2132005/1

Member of these Collections

• iGEM Parts Registry