Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Livia Seno Ferreira Camargo
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-18 05:31:00
Codon optimized mCherry for Chlamydomonas reinhardtii
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K2136016_sequence (Version 1) |
Description
When working with molecular biology a frequently asked questions is: ???How well expressed is my protein being????. Then, after all the hard process of cultivation, separation and purification comes the answer: ???Not very good??? and then comes all the questions ???WHAT???S HAPPENING HERE?! Were cells cloned properly? Did I choose the wrong clone? Was the protein analysis appropriate? Or is it just universe conspiration????Things are even worse when working with microalgae like Chlamydomonas reinhardtii because while E. coli takes 20 minutes to duplicate, C. reinhardtii takes 6...HOURS!
Trying to help you solve all this questions, the USP/UNIFESP-Brazil team has developed a special device for everyone intending to work and understanding those little green beauties. This device is able to visually differ good and bad clones, give a qualitative idea of gene expression or promoter activity, characterize protein-protein interaction and signal transduction. As if it were not enough, the kinetics of the process can be monitored in real-time with aliquots of the cultivation medium or the biological material in study.