Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chern Juin Ong, Ciara McCarthy
Date created: 2016-08-11 11:00:00
Date modified: 2016-10-04 06:11:25
psaA1 promoter + 5
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K2148000_sequence (Version 1) |
Description
Promoter and 5'UTR sequence for the psaA gene in Chlamydomonas reinhardtii chloroplast, flanked with the Phytobrick fusion sites for a promoter with 5'UTR part. Submitted in the universal acceptor vector (psB1C3), and can be extracted using Bsa1 to give the necessary Phytobrick fusion site overhangs for Golden Gate assembly to produce a transcriptional unit with other Phytobricks.Notes
Added Phytobrick fusion sites to flank promoter 5'UTR sequenceSource
Chlamydomonas reinhardtii chloroplast genome| Sequence Annotation | Location | Component / Role(s) |
| Phytobrick promoter prefix fusion site psaA1 promoter Phytbrick 5'UTR to CDS fusion site 5'UTR | 1,4 5,149 284,287 150,283 | engineered_region feature/BioBrick feature/promoter promoter engineered_region feature/BioBrick sequence_feature feature/misc |