BBa_K2152006

BBa_K2152006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2152006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MingZhe Chen
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 08:41:41

T7 promoter + RBS + killerred



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K2152006_sequence (Version 1)

Description

We add an T7 promoter in front of the killer red, and use IPTG to induce and then test the expression of killer red.
In our project, we design this part to make it more easy to test.

Notes

The point mutation is taken to avoid the XbaI error cut

Source

killer red is from the plate 1 of 2016 distribution plates. T7 and RBS is from the pet28a vector.

Sequence Annotation Location Component / Role(s)
BBa_K1184000
T7 promoter
RBS
88,804
11,29
30,54
feature/BioBrick engineered_region
promoter feature/promoter
feature/rbs ribosome_entry_site
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2152006/1