BBa_K216008

BBa_K216008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K216008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Edinburgh iGEM 2009

Date created: 2009-10-15 11:00:00

Date modified: 2015-05-08 01:11:30

bacterial luciferase LuxAB of Xenorhabdus luminescens

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K216008_sequence (Version 1)

Description

This part includes the coding sequences of luxA and luxB of Xenorhabdus luminescens, with their native ribosome binding sites. The dimeric bacterial luciferase LuxAB produces light in the presence of oxygen, FMNH2, and a long chain aldehyde. The aldehyde can be provided by the luxCDE gene products, but if these are not present, n-decanal can be added to cultures.

Notes

No special considerations. Please note that preliminary sequencing suggests that there may be a problem with the SpeI site of the construct submitted. If this is confirmed, we will submit a revised version and remove this note.

Source

Amplified from genomic DNA of Xenorhabdus luminescens.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rbs	15,20	ribosome_entry_site, feature/rbs
start luxA	27,29	feature/start, start_codon
end luxA	1107,1109	feature/stop, stop_codon
luxA coding sequence	27,1109	CDS, feature/cds
rbs	1112,1117	ribosome_entry_site, feature/rbs
start luxB	1124,1126	feature/start, start_codon
end luxB	2105,2107	feature/stop, stop_codon
luxB coding sequence	1124,2107	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K216008/1

Member of these Collections

• //function/reporter/light
• iGEM Parts Registry