Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Edinburgh iGEM 2009
Date created: 2009-10-15 11:00:00
Date modified: 2015-05-08 01:11:30
PyeaR with firefly luciferase
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K216015_sequence (Version 1) |
Description
This is the nitrate- and nitrite-responsive promoter PyeaR of Escherichia coli with the luciferase of firefly Photinus pyralis (deposited by Slovenia iGEM 2007) with the addition of a strong bacterial ribosome binding site. It should generate light in the presence of nitrate/nitrite and luciferin.Notes
No special considerations. Note that sequencing shows an unexpected insertion of 6 bases, ACCACC, after the scar between the ribosome binding site and the ATG of the luciferase coding sequence; that is, the sequence reads ACTAGACCACCATG rather than ACTAGATG. If this is correct, these 6 bases must be present in luciferase BioBrick BBa_I712019, or else were introduced by a random event during construction. This makes the RBS 12 bases from the ATG, somewhat more than the optimum, but with a strong RBS (like J15001) should still give about 25% of the expression level expected for optimum spacing in E. coli (Vellanoweth & Rabinowitz, 1992, Molecular Microbiology 6, 1105-1114).Source
Made by joining PyeaR BioBrick BBa_K216005 to a PCR fusion product of strong synthetic ribosome binding site J15001 and firefly luciferase BioBrick BBa_I712019.| Access | Instance | Definition |
| public public public | BBa_K216005 BBa_J15001 BBa_I712019 | BBa_K216005 BBa_J15001 BBa_I712019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K216005 BBa_J15001 BBa_I712019 | 1,100 109,118 125,1777 | BBa_K216005 BBa_J15001 BBa_I712019 |