BBa_K2172002

BBa_K2172002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2172002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bowen Xiao
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 08:15:40

Tac Promoter-RBS-GST-Thrombin Protease-TEV-GFP-



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Intermediate

    Sequences BBa_K2172002_sequence (Version 1)

    Description

    This part contains SmCPS1 gene, TEV cleavage site and GFP. It can be used to construct further parts that can synthesize SmCPS1.

    Notes

    Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.

    Source

    The source of the part is the vector pGEX-KG and Salvia miltiorrhiza

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2172002/1
     

    Attachments

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