BBa_K2172005

BBa_K2172005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2172005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bowen Xiao
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-18 04:09:49

Tac Promoter-RBS-GST-Thrombin Protease-GFP



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2172005_sequence (Version 1)

Description

This part is a gene circuit that can be used to test whether it is being expressed or not. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin restriction enzyme cleavage site plus a TEV restriction enzyme cleavage site (for adding other segments) and a GFP detection unit. When transformed into bacteria, e.g. E. coli, it can be used to test whether the bacteria are expressing the part not. Other parts, such as the gene SmCPS1, can be inserted into this part via digestion on the thrombin restriction enzyme cleavage site and the TEV site.

Notes

Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.

Source

The source of the part is the vector pGEX-KG.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2172005/1