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BBa_K2173002

BBa_K2173002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2173002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chandel Angad Singh

Date created: 2016-10-20 11:00:00

Date modified: 2016-10-21 06:28:09

LuxI_LuxR_mrfp1(plux)

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K2173002_sequence (Version 1)

Description

luxI with LVA degradation tag expressed under Plux promoter and a double terminator + LuxR expressed under constitutive promoter J23100 and double terminator + mRFP1 with DAS Degradation tag expressed under Plux-lambda promoter and double terminator

Notes

We used LuxI ,LuxR and mRFP1 with Deg tags and after each part there was a double terminator.

Source

Vibrio fischeri,

Components

Access	Instance	Definition
public public public public public public public public public public public public	BBa_R0062 BBa_B0030 BBa_C0061 BBa_B0015 BBa_J23100 BBa_B0030 BBa_C0062 BBa_B0015 BBa_K415032 BBa_B0030 BBa_E1010 BBa_B0015	lux pR BBa_B0030 luxI BBa_B0015 BBa_J23100 BBa_B0030 luxr BBa_B0015 BBa_K415032 BBa_B0030 mRFP1 BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0062 BBa_B0030 BBa_C0061 BBa_B0015 BBa_J23100 BBa_B0030 BBa_C0062 BBa_B0015 BBa_K415032 BBa_B0030 BBa_E1010 BBa_B0015	1,55 64,78 85,702 736,864 873,907 916,930 937,1692 1726,1854 1863,1930 1939,1953 1960,2665 2674,2802	lux pR BBa_B0030 luxI BBa_B0015 BBa_J23100 BBa_B0030 luxr BBa_B0015 BBa_K415032 BBa_B0030 mRFP1 BBa_B0015