Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sofiane Safi-Stibler
Date created: 2016-08-04 11:00:00
Date modified: 2016-10-13 10:58:33
PFur - Promoter Fur for B. subtilis
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K2180007_sequence (Version 1) |
Description
This part is made of PleiaG promoter, a fur (Ferric Uptake Regulator) binding sites and a RBS. There is a ClaI restriction site before the Fur Box (Fur Binding Site) and a PscI restriction site after. They allows us to remplace Fur Box by another operating sequence. A BamHI restriction sites is present before the PliaG sequence that may be used to insert the whole sequence. We use this part in our project to promote the LacI + LVA expression under the control of Fur.Notes
We had to check the compatibility of the promoter with the Fur Box.Source
This part is based on a previous IGEM part (add part). We add the Fur Box site we found (add site)| Sequence Annotation | Location | Component / Role(s) |
| BamHI PliaG ClaI Fur Box PscI RBS NcoI | 1,6 16,136 137,142 143,163 164,169 170,180 188,193 | feature/misc sequence_feature feature/promoter promoter sequence_feature feature/misc feature/binding non_covalent_binding_site sequence_feature feature/misc ribosome_entry_site feature/rbs sequence_feature feature/misc |