Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Margalit Mualem
Date created: 2016-09-29 11:00:00
Date modified: 2016-09-30 09:38:02
mutant SNP for non taster coffee bitterness taste and guidRNA
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K2184036_sequence (Version 1) |
Description
The basic assumption of the project is based on the fact that there is a direct link between particular taste affection and the number of receptors for that taste. For this reason, the ability to control the polymorphism of taste receptors may be useful in many ways to human society, for example, specific desirable diet aid by reducing the ability to feel a specific unhealthy taste, reducing alcohol consumption by changing sensitivity to alcohol, reducing the risk of high blood pressure by eliminating the need for salt taste, changing the sensitivity for bitterness to allow swallowing a pill for the purpose of providing medical care and more.This molecule was specially designed so that it will able to identify SNPs in one specific flavor-coffee bitterness. flavors respectively and mediate CRISPR editing of the non-taste alleles only.
Engineered SNP (Single Nucleotide Polymorphism) in favor of this reaction. This SNP enables identification of a certain taste out of the five known senses of taste, Each of the SNP???s that were selected Refer to people that unables identification of a certain taste out of the five known senses of taste.
Using SNP segments ??? the Taqman reaction makes it possible to identify the presence of a specific allele tested for a specific taste. Whenever a sample DNA is tested a presence of a specific allele is checked by one of the 15 SNP's we receive a fluorescence signal due to the release of the PROBE and vice versa. 67 DNA samples were tested using this method (samples were collected from 67 students and teachers from the school). Each sample was tested to check the presence of alleles to identify different tastes in accordance to the sequence of the SNP.
ThIs molecule WAS specially designed so that it was able to identify SNPs in specific allele flavor respectively and mediate CRISPR editing of the non-taste alleles only.
Notes
Engineered mutant SNP for non taster coffee bitterness taste by swich T with G base.Engineered guidRNA to this SNP using a tool called Crispr.MIT.Edu. guide wAS the same for SNP. the SNP made enough of a difference to create or destroy a PAM site. as Cas9 would cut one kind and not the other (PAM is NGG, so if one SNP had NGG and one had NGC as a SNP,We engineered a guide upstream to this PAM and expect Cas9 to cut the NGG form and not the NGC.)
Source
Engineered mutant SNP for non taster coffee bitterness taste and guidRNA.| Access | Instance | Definition |
| public public | BBa_K2184002 BBa_K2184033 | BBa_K2184002 BBa_K2184033 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2184002 BBa_K2184033 | 1,20 29,209 | BBa_K2184002 BBa_K2184033 |