Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marissa Sumathipala
Date created: 2016-09-03 11:00:00
Date modified: 2016-09-04 04:52:20
Penicilium alpha amylase w/out promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2185000_sequence (Version 1) |
Description
This composite part contains a genetic construct for the expression of the Pencilium alpha amylase gene, but does not contain a promoter. This enzyme has the ability to break alpha bonds between starch molecules and can thus be used for starch degradation.Components:
Kozak Sequence Part:BBa_K165002
Secretion Tag Part:BBa_K792002
Alpha Amylase Coding Sequence
ADH1 Terminator BBa_K392003
Notes
We had to include the bioBrick Prefix and suffix. A Kpn restriction enzyme site was added after the biobrick prefix, for insertion into the plasmid we chose to use. All Kpn and Spe restriction enzyme sites were removed from this sequence using Gene Design's online tool. The sequence was also codon optimized for S.cerevisiae using Gene Design.Source
The coding sequence for the amylase enzyme originates from the fungus Penicilium. The actual sequence was taken from NIH's genomic database, GenBank.| Access | Instance | Definition |
| public public public | BBa_K165002 BBa_K392003 BBa_K792002 | BBa_K165002 BBa_K392003 MFa1-Secre |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K165002 BBa_K392003 BBa_K792002 | 1,18 27,155 164,217 | BBa_K165002 BBa_K392003 MFa1-Secre |