Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Broad Run iGEM Team
Date created: 2016-09-04 11:00:00
Date modified: 2016-09-15 01:55:03
S.ferax alpha amylase w/ cyc promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2185007_sequence (Version 1) |
Description
This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the fungus S.ferax that codes for an alpha amylase enzyme. This composite part contains a constitutive promoter, coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. The amylase enzyme can be expressed by cloning this part into a plasmid without a promoter built in (such as the S.cerevisiae vector pRS426).Notes
Codon optimized for S.cerevisiae, EcoR1 and Spe1 sites removed.Source
The coding sequence of this part comes from the fungus S.ferax| Access | Instance | Definition |
| public public public public public | BBa_I766555 BBa_K165002 BBa_K792002 BBa_K2185006 BBa_K392003 | BBa_I766555 BBa_K165002 MFa1-Secre BBa_K2185006 BBa_K392003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I766555 BBa_K165002 BBa_K792002 BBa_K2185006 BBa_K392003 | 1,244 253,270 279,332 339,1743 1752,1880 | BBa_I766555 BBa_K165002 MFa1-Secre BBa_K2185006 BBa_K392003 |