BBa_K220003

BBa_K220003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K220003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarah Sandock

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:31

ADP - sugar Pyrophosphatase (NudF)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K220003_sequence (Version 1)

Description

nudF is a plasmid gene (560 bp) that codes for the protein responsible for the enzymatic reaction in mevalonent pathway to form Dimethylallyl pyrophosphate (DMAPP). Specifically, in our project, we used it in the last step of mevalonate pathway to produce biofuels iso-pentenol. We PCR amplified gene nudF with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.

Reference: ST Withers and JD Keasling, Biosynthesis and engineering of isoprenoid small molecules, http://www.springerlink.com/content/p118kp4816486248/fulltext.html

Notes

Length: 53
Fwd: 5'- CGA TCT AGA AGG AGG ATA TAT TAA TGA AAT CAT TAG AAG AAA AAA CAA TTG CC -3'

Length: 38
Rev: 5'- TCA CTG CAG ATC ACT AGT TCA TTT TTG TGC TTG GAG CG -3'

Source

Gene isolation E.Coli Strain K12

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K220003/1

Member of these Collections

• iGEM Parts Registry