BBa_K220005

BBa_K220005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K220005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarah Sandock

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:31

Methionine Kinase

Details

• Types: DnaRegion
• Roles: Coding; CDS

Description

metK is a plasmid gene (1200 bp) that codes for the enzyme that catalyzes the reaction which produces a methyl donor which is used in the conversion of Glycine to Glycine betaine. The enzyme for this reaction is S-adenosyl-methionine (SAM) synthetase. S-adenosyl-methionine produced from Methionine and one ATP molecule in the presence of SAM synthetase. Three SAM molecules are used in the methylation of Glycine to Glycine betaine (trimethyl glycine). In our project Glycine betaine (an osmoprotectant) was synthesized in E. coli to increase salt tolerance. It was hypothesized that an over expression of metK would lead to an increased production of Glycine betaine.


References: Y. Wei and E.B. Newman, Studies on role of metk gene product of Escherichia coli K 12, Molecular Microbiology (2002)

Notes

Length: 38
Fwd: 5'- CGA TCT AGA AGG AGG ATA TAT TAA TGT CCC GCC GTC TC -3'
Length: 33
Rev: 5'- TCA CTG CAG ATC ACT AGT TCA GTG AAC AAG CGG-3'

Source

Gene isolated from Streptomyces Spectabilis

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K220005/1

Member of these Collections

• iGEM Parts Registry