BBa_K227006

BBa_K227006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K227006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: WashU iGEM 2009
Date created: 2009-10-11 11:00:00
Date modified: 2015-05-08 01:11:32

puc BA coding region of R. sphaeroides



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K227006_sequence (Version 1)

Description

puc BA is the complete LHII transmembrane protein coding region found naturally in Rhodobacter Sphaeroides. LHII complexes form the light-harvesting antennae the funnel energy down to LHI and the reaction center. They are naturally found in the ratio of 3-6.7 LHII per LHI. Data suggests the precence of puc C,D, and E regions also contribute to the formation of a functional protein.

Notes

puc A and puc B together create the antennae complex necessary to harvest varying intensities of light. A common problem in bioreactors is the uneven distribution of light between cells nearest the light source and cells farther away from the light source. One way of combating this problem is modify the antenna size of the organism. By truncating the light-harvesting antenna, cells at the exterior of the bioreactor waste less light through Non-Photochemical Quenching, thus allowing photons to penetrate deeper and reach cells in the interior of the bioreactor. This leads to an increase in photosynthetic efficiency and productivity for the entire culture.

Source

PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter.

Sequence Annotation Location Component / Role(s)
pucB
RBS
pucA
1,156
159,163
172,336
CDS feature/cds
feature/binding non_covalent_binding_site
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K227006/1