Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan Shen
Date created: 2009-09-05 11:00:00
Date modified: 2015-05-08 01:11:32
Bistable part
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K228003_sequence (Version 1) |
Description
It is a part which has two stable states--CI and CI434 and the orginal state can be either of the two. CI repressor is expressed after a CI434 repressible promoter, while the CI434 is expressed after a CI repressible promoter, thus the system can only stay in one state, when one is expressed and the other is repressed. The two states can be swicthed in certain conditions. In other words, the orginal state can be changed by exogenetic triggering. For example, the orginal state is CI, then you can use an gene which exogenetically expresses CI434 to trigger the bistable part into the state of CI434.Source
The part is made up of the standard parts from partsregistry, and some of the sub-parts are inverted by PCR.| Sequence Annotation | Location | Component / Role(s) |
| mRFP eGFP CI434 repressor rbs lamda CI repressor rbs rbs CI regulated promoter rbs CI434 OR1 CI434 regulated promoter | 1,681 2219,2938 1561,2196 2203,2211 707,1420 688,700 1426,1432 1491,1539 1546,1554 1439,1454 1439,1490 | CDS feature/cds feature/cds CDS CDS feature/cds ribosome_entry_site feature/rbs CDS feature/cds feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs promoter feature/promoter feature/rbs ribosome_entry_site feature/binding non_covalent_binding_site promoter feature/promoter |