BBa_K228004

BBa_K228004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K228004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lin Min

Date created: 2009-09-17 11:00:00

Date modified: 2015-05-08 01:11:32

NahR( reverse) - salicylate promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K228004_sequence (Version 1)

Description

This is a salicylate inducible promoter part.
The activator protein is right upstream but in a opposite direction of the regulated promoter.
In the presence of salicylate, the promoter can be activated.

Notes

There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression.

Source

It is constrcuted by PCR from the plasmid kindly provided by Voigt Lab in UCSF.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
NahR	1,903	feature/cds, CDS
Pr promoter	904,993	promoter, feature/promoter
pSal	982,1050	feature/promoter, promoter

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K228004/1

Member of these Collections

• iGEM Parts Registry