Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lin Min
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:34
pSB1A2-lacZ screening plasmid
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K228867_sequence (Version 1) |
Description
T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.Notes
when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.Source
part registery 2009 distribution & PKU 2009 igem team| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 conserved BBa_B0034 START lacZ STOP BBa_I732006 pSB1A2 Backbone | 1,35 48,51 44,55 62,64 62,295 290,295 62,295 303,2366 | feature/BioBrick engineered_region conserved_region feature/conserved engineered_region feature/BioBrick feature/start start_codon feature/cds CDS stop_codon feature/stop feature/BioBrick engineered_region feature/misc sequence_feature |