BBa_K228870

BBa_K228870 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K228870
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoqian Zhang
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:34

AND GATE AraC+RBS(B0030)+T7ptag+Sal+SupD



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K228870_sequence (Version 1)

Description

{{PKU_Beijing2009_AND_GATE_FAMILY}}
This is an AND GATE in Ecoli. Use salicylate to induce the transcription of SupD and use arabinose to induce the transcription of T7ptag. If the SupD tRNA exists, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.

This gate is only one of nine gates(K228870-K228878), which are distinguished by the RBS of T7ptag.

For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133

Source

It is made up of standard subparts from the partregistry.

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