BBa_K239001

BBa_K239001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K239001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Axel Nystrom

Date created: 2009-06-21 11:00:00

Date modified: 2015-05-08 01:11:36

Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K239001_sequence (Version 1)

Description

Sequence contains 2 phosphorylated BaeR binding sites, 1 phosphorylated CpxR binding site and transcription is initiated by sigma factor 70.
Function: Can detect various periplasmic stresses.
Area of application: Hopefully it can be used to detect presence of relevant unfolded proteins in the periplasmic space, to detect shear stress or other so far undefined relevant stress.

Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first BaeR binding site and ends 5 pb after the -10 box.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10	150,157	promoter, feature/promoter
-35	128,136	promoter, feature/promoter
BaeR	4,22	non_covalent_binding_site, feature/binding
BaeR	66,81	non_covalent_binding_site, feature/binding
CpxR	107,120	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K239001/1

Member of these Collections

• iGEM Parts Registry