BBa_K243014

BBa_K243014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K243014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-13 11:00:00
Date modified: 2015-05-08 01:11:37

His-FluA-Middle Linker-Fok_i



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K243014_sequence (Version 1)

Description

These part units some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain foka, make it possible to purifier and detect the function of the protein in one step. The fluorescineA tag allows the measurement by quenching and the coupling to a anticalin linked oligo.

Notes

The choice of the linker, lipocalin tag and purification tag allows us several different combinations.

Source

Combined the parts by serial cloning steps.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K243014/1