Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Romain Bodinier
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:41
FecA mutated - constitutive activity of the fec operon
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K257019_sequence (Version 1) |
Description
FecA is a protein that binds the ferric citrate and enabled a transduction pathway that leads to the expression of the fec operon.We designed it to have a constitutive activity.
Notes
The original sequence contained 1 Pst1 site and required two mutations to obtain its constitutive activity.Source
K12 genomic DNA| Sequence Annotation | Location | Component / Role(s) |
| good rbs fecA double codon stop W706P site pstI removed | 1,8 14,2335 2336,2341 374,376 287,292 | feature/rbs ribosome_entry_site CDS feature/cds stop_codon feature/stop sequence_alteration feature/mutation sequence_alteration feature/mutation |