Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Petros Mina
Date created: 2009-09-08 11:00:00
Date modified: 2015-05-08 01:11:42
GFP-Terminator
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K259006_sequence (Version 1) |
Description
Part Description
This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator.
Green Fluorescent protein (originally from part BBa_E0040) that will glow green when excited by the correct wavelengths and thus can be used as a reporter gene.
This part is directly fused to a terminator (originally from part BBa_B0014) so it is ideal to be fused at the end of your construct.
Table of Statistics
{| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2"
!Chassis
!Monomer/Multimer
!Aminoacid Length/Weight
!Localisation
!Structure
!Excitation wavelengths
!Emmision wavelengths
|-
|Prokaryotic and Eukaryotic
|Bipartite
|274 aminoacids
|
|beta-barrel(for GFP) hairpins for Terminator
|501nm
|511nm
|}
References
#Valdivia pmid=8707053
Notes
No additional design considerations. The parts were fused together following RFC10 assembly.Source
GFP originates from jellyfish Aequeora victoria. For more details visit the SwissProt entry.The double terminator was designed by Reshma Shetty. For more details visis the original part page.