Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Petros Mina
Date created: 2009-09-08 11:00:00
Date modified: 2015-05-08 01:11:42
AraC Promoter fused with RBS
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K259007_sequence (Version 1) |
Description
This part has the arabinose regulated promoter (AraC/pBAD) upstream of a ribosome binding site. This is ideal for inserting a protein to be expressed downstream of this part as it's expression can be regulated by the presence of the concentration of arabinose in the growing environment/media of the cells.What this part actually does
This composite part includes the AraC/pBAD promoter and a strong ribosome binding site designed from the Anderson family of RBS.
The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins.
When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the trancription of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth.
Controlling the transcription will not ensure translation. The ribosome binding site is ideally placed a small distance away from the promoter site to ensure that the ribosome will bind and start translating the protein coding sequence found downstream.
Notes
The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.Source
The AraC promoter can be found naturally at 1.52min of the E.coli genome (EcoGene number EG10054.The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page here.
| Access | Instance | Definition |
| public public public | BBa_R0081 BBa_R0080 BBa_J61100 | AraC O2 AraC BBa_J61100 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0081 BBa_R0080 BBa_J61100 | 1,183 192,340 349,360 | AraC O2 AraC BBa_J61100 |