Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-16 11:00:00
Date modified: 2015-05-08 01:11:42
pCC2FosM
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K260000_sequence (Version 1) |
Description
This is a fosmid based on pCC2Fos from EPICENTRE Biotechnologies (http://www.epibio.com/item.asp?ID=385). It contains 38 kb from the human MECP2 genomic locus, with a spectinomycin resistance (SpecR) cassette in the first intron of MECP2.This fosmid is used to accept by recombineering the HincII fragment from BBa_K260016 (inside backbone BBa_K260010). This reaction also transfers the blasticidin resistance gene (BsdR) to generate the new backbone BBa_K260001 (pCC2FosMB), then containing the same BBa_K260016 part.
Notes
Intron 1 of MECP2 previously contained a BsdR cassette. This had to be changed to SpecR by recombineering to generate the present fosmid.Source
EPICENTRE Biotechnologies (http://www.epibio.com/item.asp?ID=385)human MECP2 genomic sequence.
| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter Start of human MECP2 genomic sequence End of human MECP2 genomic sequence SpecR CmR selection cassette | 311,329 383,383 39898,39898 35631,36305 40335,40994 35196,36374 | promoter feature/promoter start_codon feature/start feature/stop stop_codon CDS feature/cds CDS feature/cds sequence_feature feature/misc |